Post Doctoral Fellow (Human Frontier Science Program)
Department of Chemical Engineering
Massachusetts Institute of Technology
77 Massachusetts Avenue, Room: E18-524
Cambridge, MA 02139 USA
Phone: (617) 258-8037
Email: khasling@mit.edu
Education
Heidelberg University (Ruperto Carola), 2014
Heidelberg, Germany
Diploma (eqiv. to B. Sc and M. Sc) Biology, 2010
Julius Maximilian University
Würzburg, Germany
Research Interests
I am interested in the construction of recombinant pathways for the fermentative production of natural products. My main focus is on designing microbes that produce plant secondary metabolites, which are valuable precursors for the chemical and pharmaceutical industries.
Publications
Peschke M*, Haslinger K*, Brieke C, Reinstein J, Cryle MJ (2016) Regulation of the P450 oxygenation cascade involved in glycopepetide antibiotic biosynthesis. JACS 138: 6746-6753.
Haslinger K, Cryle MJ (2016) Structure of OxyAtei : completing our picture of the glycopeptide antibiotic producing Cytochrome P450 cascade. FEBS Lett 590: 571-581.
Brieke C, Peschke M, Haslinger K, Cryle MJ (2015) Sequential in vitro cyclization by cytochrome P450 enzymes of glycopeptide antibiotic precursors bearing the X-domain from nonribosomal peptide biosynthesis. Angew Chemie – Int Ed 54: 15715-15719.
Haslinger K*, Peschke M*, Brieke C, Maximowitsch E, Cryle MJ (2015) X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Nature 521: 105–109.
Haslinger K, Redfield C, Cryle MJ (2015) Structure of the terminal PCP domain of the non-ribosomal peptide synthetase in teicoplanin biosynthesis. Proteins 83: 711–721.
Brieke C, Kratzig V, Haslinger K, Winkler A, Cryle MJ (2015) Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Org Biomol Chem 13: 2012–2021.
Haslinger K, Maximowitsch E, Brieke C, Koch A, Cryle MJ (2014) Cytochrome P450 OxyBtei Catalyzes the First Phenolic Coupling Step in Teicoplanin Biosynthesis. ChemBioChem 15: 2719–2728.
Haslinger K, Brieke C, Uhlmann S, Sieverling L, Süssmuth RD, Cryle MJ (2014) The structure of a transient complex of a nonribosomal peptide synthetase and a cytochrome P450 monooxygenase. Angew Chemie – Int Ed 53: 8518–8522.
Cryle MJ, Brieke C, Haslinger K (2013) Oxidative transformations of amino acids and peptides catalysed by Cytochromes P450. In Farkas, Etelka and Ryadnov M (ed.), Amino Acids, Peptides and Proteins pp 1–36. The Royal Society of Chemistry, Cambridge.
*These authors contributed equally to this work.