Publications 1998 – 2008


Leonard, E., Nielsen, D., Solomon, K., and Prather, K. L. J. Engineering microbes with synthetic biology frameworks. Trends Biotechnol. 26(12): 674-681 (2008) DOI:10.1016/j.tibtech.2008.08.003

Rodrigo, G., Carrera, J., Prather, K.L.J., and Jaramillo, A. DESHARKY: Automated design of metabolic pathways for optimal cell growth. Bioinformatics. 24(21): 2554-2556 (2008). DOI:10.1093/bioinformatics/btn471

Prather, K.L.J. and Martin, C.H. De novo biosynthetic pathways: rational design of microbial chemical factories. Curr. Opin. Biotechnol. 19(5): 468-474 (2008). DOI:10.1016/j.copbio.2008.07.009


Prather, K. L. J., Edmonds, M. C., and Herod, J. W. Identification and characterization of IS1 transposition in plasmid amplification mutants of E. coli clones producing DNA vaccines. Appl. Micrbiol. Biotechnol. 73:815-826 (2006).

Montgomery, D. L., and Prather, K. J. “Design of plasmid DNA constructs for vaccines.” in DNA Vaccines: Methods and Protocols (eds. Saltzman, W.M., Shen, H. & Brandsma, J.L.), 2nd Ed., Humana Press, Totowa, NJ, 2006.


Prather, K. J., Sagar, S., Murphy, J., and Chartrain, M. Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification. Enz. Microbiol. Technol. 33: 865-883 (2003). DOI:10.1016/S0141-0229(03)00205-9


Aldor, I.S., Kim, S.-W., Prather, K.L.J., and Keasling, J.D. Metabolic engineering of a novel propionate-independent pathway for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Salmonella enterica serovar Typhimurium. Appl. Envir. Microbiol. 68: 3848-3854 (2002).


Jones, K.L., Kim, S.-W., and Keasling, J.D. Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Metabolic Engineering. 2: 328-338 (2000).DOI: 10.1006/mben.2000.0161


Carrier, T.A., Jones, K.L., and Keasling, J.D. mRNA stability and plasmid copy number effects on gene expression from an inducible promoter system. Biotechnol. Bioeng. 59: 666-672 (1998). DOI: 10.1002/(sici)1097-0290(19980920)59:6<666::aid-bit2>;2-d

Jones, K.L. and Keasling, J.D. Construction and characterization of F plasmid-based expression vectors. Biotechnol. Bioeng. 59: 659-665 (1998).