Publications: 2012-2014


Sheppard, M.J., A.M. Kunjapur, S.J. Wenck, and K.L.J. Prather. 2014. “Retro-biosynthetic screening of a modular pathway design achieves selective route for microbial synthesis of 4-methyl-pentanol.” Nat. Commun. 5 5031.

Kunjapur, A.M., Y. Tarasova, and K.L.J. Prather. 2014. “Synthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli.” J. Am. Chem. Soc. 136(33): 11644-11654.
doi: 10.1021/ja506664a

Gonçalves, G.A.L., K.L.J. Prather, G.A. Monteiro, D.M.F. Prazeres. 2014. “Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges.” Vaccine 32(24): 2847-2850.
doi: 10.1016/j.vaccine.2014.02.023

Simcikova, M., K.L.J. Prather. D.M.F. Prazeres and G.A. Monteiro. 2014. “On the dual effect of glucose during production of pBAD/AraC-based minicircles.”  Vaccine 32(24): 2843-2846.
doi: 10.1016/j.vaccine.2014.02.035

Hicks, M.A. and K.L.J. Prather. 2014.  “Bioprospecting in the Genomic Age.” In Sima Sariaslani, Geoffrey Gadd, editors: Advances in Applied Microbiology, Vol 87, AAM, UK: Academic Press, pp. 111-146.
doi: 10.1016/B978-0-12-800261-2.00003-7

Gonçalves, G.A.L., K.L.J. Prather, G.A. Monteiro, A.E. Carnes, D.M.F. Prazeres.  2014. “Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing.”  J. Biotechnol. 186: 119-127.
doi: 10.1016/j.jbiotec.2014.06.008

Shiue, E. and K.L.J. Prather. 2014.  “Improving D-glucaric acid production from myo-inositol in E. coli by increasing MIOX stability and myo-inositol transport.”  Metab. Eng. 22:22-31.
doi: 10.1016/j.ymben.2013.12.002

McMahon, M.D. and K.L.J. Prather. 2014. “Functional screening and in vitro analysis reveals thioesterases with enhanced substrate specificity profiles that improve medium-chain fatty acid production titers in Escherichia coli.” Appl. Environ. Microbiol. 80(3):1042-1050.

Abin-Fuentes, A., J. C. Leung, M. E. Mohamed, D.I.C. Wang and K.L.J. Prather. 2014. “Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system.”  Biotechnol. Bioeng. 111(5):876-884.
doi: 10.1002/bit.25148

Dhamankar, H., Y. Tarasova, C.H. Martin, and K.L.J. Prather. 2014. “Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) as a value added chemical from glucose as a sole carbon source.” Metab. Eng. 25:72-81.
doi: 10.1016/j.ymben.2014.06.004

Rodrigues, J.L., M. Sousa, K.L.J. Prather, L.D. Kluskens, L.R. Rodrigues. 2014. “Selection of Escherichia coli heat shock promoters towards their application as stress probes.”  J. Biotechnol. 188:61-71.
doi: 10.1016/j.jbiotec.2014.08.005


Pirie, C., M. De Mey, K.L.J. Prather and P.K. Ajikumar. 2013. “Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.” ACS Chem. Biol. 8:662-672. doi: 10.1021/cb300634b

Abin-Fuentes, A., M.E-S. Mohamed, D.I.C. Wang, and K.L.J. Prather. 2013. “Exploring the mechanism of biocatalyst inhibition in microbial desulfurization.” Appl. Environ. Microbiol. 79:24 7546.
doi: 10.1128/AEM.03415-13

Martin, C. H.*, H. Dhamankar*, H.C. Tseng, M.J. Sheppard, C.R. Reisch, K.L.J. Prather. 2013. “A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone.” Nat. Commun. 4:1414. *Authors contributed equally to this work.
doi: 10.1038/ncomms2418

Solomon, K.V., T.S. Moon, B. Ma, T.M. Sanders, and K.L.J. Prather. 2013.  “Tuning primary metabolism for heterologous productivity.” ACS Synth. Biol. 2(3): 126-135. doi: 10.1021/sb300055e

Gonçalves, G.A.L., D.M.F. Prazeres, G.A. Monteiro, K.L.J. Prather. 2013. “De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production.” Appl. Microbiol. Biotechnol. 97(2): 611-620. doi: 10.1007/s00253-012-4308-5


Solomon, K.V., T.M. Sanders, and K.L.J. Prather. 2012.  “A dynamic metabolite valve for the control of central carbon metabolism.”  Metab. Eng.  14:661-671. doi: 10.1016/j.ymben.2012.08.006

Bower, D.M. and K.L.J. Prather. 2012.  “Development of new plasmid DNA vaccine vectors with R1-based replicons.”  Microb. Cell Fact. 11:107. doi:  10.1186/1475-2859-11-107

Tseng, H.-C., and K.L.J. Prather. 2012. “Controlled biosynthesis of odd-chain fuels and chemcials via engineered modular metabolic pathways.” PNAS. 109(44): 17925-17930. doi: 10.1073/pnas.1209002109

Fernandes, F.,  J. Vidigal, M. Dias, K.L.J. Prather, M.J.T. Carrondo, A.S. Coroadinha, A.P. Teixeira, P.M. Alves. 2012. “Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression.”  Biotechnol. Bioeng. 109(11): 2836-2844.  doi: 10.1002/bit.24542

Shiue, E. and K.L.J. Prather. 2012. “Synthetic biology devices as tools for metabolic engineering.”  Biochem. Eng. J. 65:82-89.
doi: 10.1016/j.bej.2012.04.006

Bower, D.M., K.S. Lee, R.J. Ram, and K.L.J. Prather. 2012.  “Fed-batch microbioreactor platform for scale down and analysis of a plasmid DNA production process.” Biotechnol. Bioeng. 109(8):1976-1986. doi: 10.1002/bit.24498

Moon, T.S., D.R. Nielsen, K.L.J. Prather. 2012.  “Sensitivity analysis of a proposed model mechanism for newly created glucose-6-oxidases.” AIChE J. 58(8): 2303-2308. doi: 10.1002/aic.12762

Gonçalves, G.L.,  D.M. Bower, D.M. F. Prazeres, G.A. Monteiro, K.L.J. Prather. 2012. “Rational engineering of E. coli strains for plasmid :biopharmaceutical manufacturing.” Biotechnol. J. 7: 251-261.
doi: 10.1002/biot.201100062

Solomon, K.V. and K.L.J. Prather. 2012. “The zero-sum game of pathway optimization: emerging paradigms for tuning gene expression.”  Biotechnol. J. 6(9): 1064-1070. doi: 10.1002/biot.201100086