Publications: 2015-2017


Haslinger, K. and K.L. J. Prather, “Pathway towards renewable chemicals.” Nat. Microbiol. 2(12):1580-1581 (2017).

Tan, S.Z. and K. L. J. Prather, “Dynamic pathway regulation: recent advances and methods of construction.” Curr. Opin. Chem. Biol. 41:28-35 (2017).

Reisch, C.R. and K.L. J. Prather, “Scarless Cas9 Assisted Recombineering (no-SCAR) in Escherichia coli, an easy to use system for genome editing.” Curr. Prot. Mol. Biol. 117:31.8.1-31.8.20 (2017).

Khlystov, N.A., W.Y. Chan, A.M. Kunjapur, W. Shi, K.L. J. Prather and B.D. Olsen. “Material properties of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartate (cyanophycin).” Polymer. 109 pp: 238-245 (2017).

Gupta, A., I.M. Brockman Reizman, C.R. Reisch and K.L. J. Prather. “Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit.” in press, Nat. Biotechnol. 35:273-279 (2017).


Sheppard, M. J.*, Kunjapur, A. M.* and K. L. J. Prather. “Modular and selective biosynthesis of gasoline-range alkanes.” Metab. Eng. 33, 28–40 (2016). *equal contribution
doi: 10.1016/j.ymben.2015.10.010

Kunjapur, A.M., B. Cervantes, K.L.J. Prather. “Coupling carboxylic acid reductase to inorganic pyrophosphatase enhances cell-free in vitro aldehyde biosynthesis.” Biochem. Eng. J. 109: 19–27 (2016).
doi:  10.1016/j.bej.2015.12.018

Tan, S.T., S. Manchester, K.L J. Prather. “Controlling central carbon metabolism for improved pathway yields in Saccharomyces cerevisiae.” ACS Syn. Biol. 5(2) pp: 116-124 (2016).

Simcikova, M., D.M. Prazeres, K.L J. Prather, G.A. Monteiro. “Towards an effective non-viral gene delivery vector.” Biotechnol. Genet. Eng. Rev. 31(1-2) pp: 82-107 (2016).

Kunjapur, A.M., J.C. Hyun, K.L J. Prather. “Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway.” Microb. Cell Fact. 15:61 (2016).

Simcikova, M., C.P.A. Alves, L. Brito, K.L J. Prather, D.M.F. Prazeres, G.A. Monteiro. “Improvement of DNA minicircle production by optimization of the secondary structure of the 5′-UTR of ParA resolvase.” Appl. Microbiol. Biotechnol. 100(15) pp: 6725-6737 (2016).

Gupta, A.L., M.A. Hicks, S.P. Manchester, K.L J. Prather. “Porting the synthetic D-glucaric acid pathway from Escherichia coli to Saccharomyces cerevisiae.” Biotechnol. J. 11(9) pp: 1201-1208 (2016).


Rodrigues, J.L., K.L J. Prather, L.D. Kluskens, L.R. Rodrigues. “Heterologous production of curcuminoids: a review.” Microbiol. Mol. Biol. Rev. 79 pp: 39-60 (2015).

Kunjapur, A. M., and K.L.J. Prather. “Microbial engineering for aldehyde synthesis.” Appl. Environ. Microbiol. 81 pp: 1892-1901. (2015).

Brockman, I.M., and K.L.J. Prather. “Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites.” Metab. Eng. 28 pp: 104-113 (2015).

Shiue, E., I.M. Brockman, K. L. J. Prather. “Improving product yields on D-glucose in Escherichia coli via alternative carbon sources.” Biotechnol. Bioeng.112(3) pp: 579-587 (2015).

Lopes, M.B., G.A.L. Gonçalves, D. Felício-Silva, K. L. J. Prather. G.A. Monteiro, D.M.F. Prazeres, C.R.C. Calado. “In-situ NIR monitoring of plasmid bioproduction processes: effect of the cultivation medium composition, the cultivation strategy and the Escherichia coli strain.” J. Chem. Technol. Biotechnol. 90(2) pp: 255-261 (2015).

Pfleger, B. F. and K. L. J. Prather. “Biological synthesis unbounded?” Nat. Biotechnol. 33, 1148–9 (2015).

Tan, S. Z., Manchester, S. and K. L. J. Prather. “Controlling central carbon metabolism for improved pathway yields in Saccharomyces cerevisiae.” ACS Synth. Biol. 5(2) pp: 116-124 (2015).

Boock, J. T., Gupta, A. and K. L. J. Prather. “Screening and modular design for metabolic pathway optimization.” Curr. Opin. Biotechnol. 36, 189–198 (2015).

Reizman, I. M. B., Stenger, A. R., Reisch, C. R., Gupta, A., Connors, N. C. and K. L. J. Prather. “Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes.” Metab. Eng. Commun. 2, 109–116 (2015).

Reisch, C. R. and K. L. J. Prather. “The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.” Sci. Rep. 5, 15096 (2015).

Brockman, I. M. and K. L. J. Prather. “Dynamic metabolic engineering: New strategies for developing responsive cell factories.” Biotechnol. J. 10, 1360–9 (2015).

Rodrigues, J. L., Araújo, R. G., K. L. J. Prather, Kluskens, L. D. & Rodrigues, L. R. “Heterologous production of caffeic acid from tyrosine in Escherichia coli.” Enzyme Microb. Technol. 71, 36–44 (2015).

Rodrigues, J.L., R. G. Araújo, K. L. J. Prather, Kluskens, L. D., Rodrigues, L. R. “Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli.” Biotechnol. J. 10(4) 599-609 (2015).

Cheng, D., R. Wang, K. L. J. Prather, Chow, I.-M. Hsing. “Tackling codon usage bias for heterologous expression in Rhodobacter sphaeroides by supplementation of rare tRNAs.” Enzyme Microbiol. Technol. 72:25-34 (2015).