Publications

Sheppard, M. J.*, Kunjapur, A. M.* and K. L. J. Prather. “Modular and selective biosynthesis of gasoline-range alkanes.” Metab. Eng. 33, 28–40 (2016). *equal contribution
doi: 10.1016/j.ymben.2015.10.010

Kunjapur, A.M., B. Cervantes, K.L.J. Prather. “Coupling carboxylic acid reductase to inorganic pyrophosphatase enhances cell-free in vitro aldehyde biosynthesis.” Biochem. Eng. J. 109: 19–27 (2016).
doi:  10.1016/j.bej.2015.12.018

Tan, S.T., S. Manchester, K.L J. Prather. “Controlling central carbon metabolism for improved pathway yields in Saccharomyces cerevisiae.” ACS Syn. Biol. 5(2) pp: 116-124 (2016).
doi:10.1021/acssynbio.5b00164

Simcikova, M., D.M. Prazeres, K.L J. Prather, G.A. Monteiro. “Towards an effective non-viral gene delivery vector.” Biotechnol. Genet. Eng. Rev. 31(1-2) pp: 82-107 (2016).
doi:10.1080/02648725.2016.1178011

Kunjapur, A.M., J.C. Hyun, K.L J. Prather. “Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway.” Microb. Cell Fact. 15:61 (2016).
doi:10.1186/s12934-016-0459-x

Simcikova, M., C.P.A. Alves, L. Brito, K.L J. Prather, D.M.F. Prazeres, G.A. Monteiro. “Improvement of DNA minicircle production by optimization of the secondary structure of the 5′-UTR of ParA resolvase.” Appl. Microbiol. Biotechnol. 100(15) pp: 6725-6737 (2016).
doi:10.1007/s00253-016-7565-x

Gupta, A.L., M.A. Hicks, S.P. Manchester, K.L J. Prather. “Porting the synthetic D-glucaric acid pathway from Escherichia coli to Saccharomyces cerevisiae.” Biotechnol. J. 11(9) pp: 1201-1208 (2016).
doi:10.1002/biot.201500563

Rodrigues, J.L., K.L J. Prather, L.D. Kluskens, L.R. Rodrigues. “Heterologous production of curcuminoids: a review.” Microbiol. Mol. Biol. Rev. 79 pp: 39-60 (2015).
doi:10.1128/MMBR.00031-14

Kunjapur, A. M., and K.L.J. Prather. “Microbial engineering for aldehyde synthesis.” Appl. Environ. Microbiol. 81 pp: 1892-1901. (2015).
doi:10.1128/AEM.03319-14

Brockman, I.M., and K.L.J. Prather. “Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites.” Metab. Eng. 28 pp: 104-113 (2015).
doi:10.1016/j.ymben.2014.12.005

Shiue, E., I.M. Brockman, K.L.J. Prather. “Improving product yields on D-glucose in Escherichia coli via alternative carbon sources.” Biotechnol. Bioeng.112(3) pp: 579-587 (2015).
doi:10.1002/bit.25470

Lopes, M.B., G.A.L. Gonçalves, D. Felício-Silva, K.L.J. Prather, G.A. Monteiro, D.M.F. Prazeres, C.R.C. Calado. “In-situ NIR monitoring of plasmid bioproduction processes: effect of the cultivation medium composition, the cultivation strategy and the Escherichia coli strain.” J. Chem. Technol. Biotechnol. 90(2) pp: 255-261 (2015).
doi:10.1002/jctb.4431

Pfleger, B. F. and K. L. J. Prather. “Biological synthesis unbounded?” Nat. Biotechnol. 33, 1148–9 (2015).
doi:10.1038/nbt.3399

Tan, S. Z., Manchester, S. and K. L. J. Prather. “Controlling central carbon metabolism for improved pathway yields in Saccharomyces cerevisiae.” ACS Synth. Biol. 5(2) pp: 116-124 (2015).
doi:10.1021/acssynbio.5b00164

Boock, J. T., Gupta, A. and K. L. J. Prather. “Screening and modular design for metabolic pathway optimization.” Curr. Opin. Biotechnol. 36, 189–198 (2015).
doi:10.1016/j.copbio.2015.08.013

Reizman, I. M. B., Stenger, A. R., Reisch, C. R., Gupta, A., Connors, N. C. and K. L. J. Prather. “Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes.” Metab. Eng. Commun. 2, 109–116 (2015).
doi:10.1016/j.meteno.2015.09.002

Reisch, C. R. and K. L. J. Prather. “The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.” Sci. Rep. 5, 15096 (2015).
doi:10.1038/srep15096

Brockman, I. M. and K. L. J. Prather. “Dynamic metabolic engineering: New strategies for developing responsive cell factories.” Biotechnol. J. 10, 1360–9 (2015).
doi:10.1002/biot.201400422

Rodrigues, J. L., Araújo, R. G., K. L. J. Prather., Kluskens, L. D. & Rodrigues, L. R. “Heterologous production of caffeic acid from tyrosine in Escherichia coli.” Enzyme Microb. Technol. 71, 36–44 (2015).
doi:10.1016/j.enzmictec.2015.01.001

Rodrigues, J.L., R. G. Araújo, K. L. J. Prather., Kluskens, L. D., Rodrigues, L. R. “Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli.” Biotechnol. J. 10(4) 599-609 (2015).
doi:10.1002/biot.201400637

Cheng, D., R. Wang, K. L. J. Prather, Chow, I.-M. Hsing. “Tackling codon usage bias for heterologous expression in Rhodobacter sphaeroides by supplementation of rare tRNAs.” Enzyme Microbiol. Technol. 72:25-34 (2015).
doi:10.1016/j.enzmictec.2015.02.003

Sheppard, M.J., A.M. Kunjapur, S.J. Wenck, and K.L.J. Prather. 2014. “Retro-biosynthetic screening of a modular pathway design achieves selective route for microbial synthesis of 4-methyl-pentanol.” Nat. Commun. 5 5031.
doi:10.1038/ncomms6031

Kunjapur, A.M., Y. Tarasova, and K.L.J. Prather. 2014. “Synthesis and accumulation of aromatic aldehydes in an engineered strain of Escherichia coli.” J. Am. Chem. Soc. 136(33): 11644-11654.
doi: 10.1021/ja506664a

Gonçalves, G.A.L., K.L.J. Prather, G.A. Monteiro, D.M.F. Prazeres. 2014. “Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges.”  Vaccine 32(24): 2847-2850.
doi: 10.1016/j.vaccine.2014.02.023

Simcikova, M., K.L.J. Prather, D.M.F. Prazeres and G.A. Monteiro. 2014. “On the dual effect of glucose during production of pBAD/AraC-based minicircles.”  Vaccine 32(24): 2843-2846.
doi: 10.1016/j.vaccine.2014.02.035

Hicks, M.A. and K.L.J. Prather.  2014.  “Bioprospecting in the Genomic Age.” In Sima Sariaslani, Geoffrey Gadd, editors: Advances in Applied Microbiology, Vol 87, AAM, UK: Academic Press, pp. 111-146.
doi: 10.1016/B978-0-12-800261-2.00003-7

Gonçalves, G.A.L., K.L.J. Prather, G.A. Monteiro, A.E. Carnes, D.M.F. Prazeres.  2014. “Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing.”  J. Biotechnol. 186: 119-127.
doi: 10.1016/j.jbiotec.2014.06.008

Shiue, E. and K.L.J. Prather. 2014.  “Improving D-glucaric acid production from myo-inositol in E. coli by increasing MIOX stability and myo-inositol transport.”  Metab. Eng. 22:22-31.
doi: 10.1016/j.ymben.2013.12.002

McMahon, M.D. and K. L. J. Prather. 2014. “Functional screening and in vitro analysis reveals thioesterases with enhanced substrate specificity profiles that improve medium-chain fatty acid production titers in Escherichia coli.” Appl. Environ. Microbiol. 80(3):1042-1050.
doi:10.1128/AEM.03303-13

Abin-Fuentes, A., J. C. Leung, M. E. Mohamed, D.I.C. Wang and K.L.J. Prather. 2014. “Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system.”  Biotechnol. Bioeng.111(5):876-884.
doi: 10.1002/bit.25148

Dhamankar, H., Y. Tarasova, C.H. Martin, and K.L.J. Prather. 2014. “Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) as a value added chemical from glucose as a sole carbon source.”  Metab. Eng. 25:72-81.
doi: 10.1016/j.ymben.2014.06.004

Rodrigues, J.L., M. Sousa, K.L.J. Prather, L.D. Kluskens, L.R. Rodrigues. 2014. “Selection of Escherichia coli heat shock promoters towards their application as stress probes.”  J. Biotechnol.188:61-71.
doi: 10.1016/j.jbiotec.2014.08.005

Pirie, C., M. De Mey, K.L.J. Prather and P.K. Ajikumar. 2013. “Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.” ACS Chem. Biol.8:662-672. doi: 10.1021/cb300634b

Abin-Fuentes, A., M.E-S. Mohamed, D.I.C. Wang, and K.L.J. Prather. 2013. “Exploring the mechanism of biocatalyst inhibition in microbial desulfurization.” Appl. Environ. Microbiol. 79:24 7546.
doi: 10.1128/AEM.03415-13

Martin, C. H.*, H. Dhamankar*, H.C. Tseng, M.J. Sheppard, C.R. Reisch, K.L.J. Prather. 2013. “A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone.” Nat. Commun. 4:1414. *Authors contributed equally to this work.
doi: 10.1038/ncomms2418

Solomon, K.V., T.S. Moon, B. Ma, T.M. Sanders, and K.L.J. Prather. 2013.  “Tuning primary metabolism for heterologous productivity.” ACS Synth. Biol. 2(3): 126-135. doi: 10.1021/sb300055e

Gonçalves, G.A.L., D.M.F. Prazeres, G.A. Monteiro, K.L.J. Prather. 2013. “De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production.”  Appl. Microbiol. Biotechnol. 97(2): 611-620. doi: 10.1007/s00253-012-4308-5

Dhamankar, H and K. L. J. Prather. 2011. “Microbial chemical factories: recent advances in pathway engineering for synthesis of value added chemicals.” Curr. Opin. Struct. Biol. 21(4):488-494. doi: 10.1016/j.sbi.2011.05.001