Resources

Prather Group OpenWetWare

Sigma DNA calculator
Melting temperature and dimer analysis

NEB double digest finder
Identify compatible restriction endonuclease pairs

RaCC
Rare Codon Calculator for E. coli

Registry of Standard Biological Parts
Collection of genetic parts

ExPASy
Bioinformatics resource portal

  • Compute pI/MW: compute isoelectic point and molecular weight of protein from amino acid sequence
  • Translate: translate DNA sequence to amino acid sequence

BRENDA
The comprehensive enzyme information system

KEGG
Kyoto Encyclopedia of Genes and Genomes

EcoCyc
Encyclopedia of E. coli

NCBI
National Center for Biotechnology Information

BioNumbers
The database of useful biological numbers

Members Only
Protocols and presentations

Prather Lab Training Videos
Introductory Video: Overview and Safety
Intro to the Prather Lab: part 1 2 3 4 5 6 7
PCR: part 1 2
Gel Electrophoresis: part 1 2 3
Electrocompetent Cells: part 1 2
Enzyme Kinetics: part 1 2 3
P1 Transduction: part 1 2 3